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91.
为进一步开辟北京地区水产职业技能鉴定培训新的工作方法,探索长期有效地运行机制,通过对获得的信息进行综合分析,提出以农民田间学校为载体的培训新模式。 相似文献
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松针粉富含多种营养成分,并且具有一定的药用价值。文章对我国松针的资源概况、松针粉的营养价值、药效及在生产上的应用情况进行概述,并对松针粉未来的研究方向进行展望。 相似文献
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抗球虫药物在畜禽业应用广泛,可预防治疗球虫病,提高家禽饲料转化率,提升肉品质。但是,随即会出现饲料交叉污染,对非目标动物产生毒性作用,动物性食品中药物残留超标等问题。因此,建立简便、快速、灵敏度高的检测方法检测动物源性食品中的抗球虫药物残留极为重要。基于抗原-抗体特异性结合原理的免疫分析检测技术能够完成快速检测,高通量筛选,具有特异性强、灵敏度高、操作简便、成本低的优点。本文综述了不同基质中抗球虫药物残留的免疫检测技术进展,重点介绍了酶联免疫吸附检测法、免疫层析法、荧光偏振免疫分析检测法、时间分辨荧光免疫分析检测法和生物传感器检测法。并对免疫检测技术在残留检测方面的发展趋势进行了展望,旨在为抗球虫药物的残留监控提供方法学上的参考,为新方法的建立提供思路。 相似文献
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Yuval Ramot Michal Steiner Netanel Amouyal Yossi Lavie Guy Klaiman Abraham J. Domb Abraham Nyska Tal Hagigit 《Journal of toxicologic pathology》2021,34(1):11
Fracture-related infections remain a leading cause of morbidity and mortality. We aimed to establish a simple contaminated radial osteotomy model to assess the efficacy of a biodegradable polymer poly(sebacic-co-ricinoleic acid) [p(SA-RA)] containing 20% w/w gentamicin. A unilateral transverse osteotomy was induced in Sprague-Dawley (SD) rats, followed by application of Staphylococcus aureus suspension over the fracture. After successfully establishing the contaminated open fracture model, we treated the rats either systemically (intraperitoneal cefuroxime), locally with p(SA-RA) containing gentamicin, or both. Control groups included non-contaminated group and contaminated groups that were either untreated or treated with the polymer alone. After 4 weeks, the bones were subjected to micro-CT scanning and microbiological and histopathology evaluations. Micro-CT analysis revealed similar changes in the group subjected to both local and systemic treatment as in the non-contaminated control group. Lack of detectable bacterial growth was noted in most animals of the group subjected to both local and systemic treatment, and all samples were negative for S. aureus. Histopathological evaluation revealed that all treatment modalities containing antibiotics were highly effective in reducing infection and promoting callus repair, resulting in early bone healing. While p(SA-RA) containing gentamicin treatment showed better results than cefuroxime, the combination of local and systemic treatment displayed the highest therapeutic potential in this model. 相似文献
99.
Kasumi Sudo Mariko Ochiai Naoyuki Aihara Noriyuki Horiuchi Atsushi Yamamoto Sachiko Matsumoto Koji Oishi 《Journal of toxicologic pathology》2021,34(2):137
Batch safety tests (BSTs) of veterinary vaccines are conducted using small laboratory animals to assure the safety of vaccines according to several criteria, including clinical signs and change in body weight. Although the latter is used as an evaluation index in BSTs, there have been no reports on the internal changes that affect body weight during the test period. Therefore, we analyzed BST via pathological examination of the tested animals. Here, BSTs were performed for 176 batches using mice and 126 batches using of guinea pigs. Most of the gross findings could be classified into four lesion types (nodules, adhesions, ascites, no apparent signs), with only one vaccine inducing lesions that could not be classified into any of these four types. Histopathological examination revealed that the reactions caused by BST were pyogenic and/or granulomatous inflammation. Nodular or adhesive lesions comprised more severe pyogenic granulomatous inflammation than ascites or cases with no apparent gross lesions. These nodular or adhesive lesions were more frequently induced by vaccines that contained an adjuvant than by vaccines that did not contain an adjuvant. The cases with “exceptional” gross findings histologically presented severe necrosis of the hematopoietic system. Additional testing showed that these “exceptional” lesions were induced when a specific type of light liquid paraffin was injected along with other vaccine additives. Our results show that body weight loss and/or lesions during BST were induced by proinflammatory properties of the tested vaccines and that BST is a sensitive method for detecting unexpected effects of vaccine components. 相似文献
100.
试验以"LAMP+微流控"技术为基础,开发饲料动物源性成分检测芯片。针对牛、羊、猪、鸡的16srRNA靶序列,根据LAMP引物设计软件进行4种肉类LAMP引物设计。在LAMP优化试验、芯片优化测试、特异性验证试验和芯片应用测试中,进行最佳引物筛选测试和特异性测试。结果显示,筛选出的4种肉类最佳引物特异性强,最佳反应温度为65℃。芯片特异性验证试验的归一化荧光曲线显示,试验结果准确、特异性稳定。对饲料市场购买的16份样本中检测出的动物源性成分,采用中华人民共和国农业行业标准验证,符合率100%。微流控检测芯片的开发与应用为饲料中动物源性成分检测提供新方法,为饲料产品质量安全提供保障。 相似文献